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A simple and efficient method for CRISPR/Cas9-induced mutant screening

April 10th, 2017

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system provides a technological breakthrough in mutant generation. Several methods such as the polymerase chain reaction (PCR)/restriction enzyme (RE) assay, T7 endonuclease I (T7EI) assay, Surveyor nuclease assay, PAGE-based genotyping assay, and high-resolution melting (HRM) analysis-based assay have been developed for screening CRISPR/Cas9-induced mutants.

Biomimetic sensors and biosensors for qualitative and quantitative analyses of five basic tastes

April 10th, 2017

As is known to all, the basic taste includes sweetness, bitterness, sourness, saltiness and umami. The core of taste evaluation is the sense of taste. Sensory evaluation and chemical detection, as the methods of evaluating flavor quality, are commonly used in many products such as food stuffs, beverages and drugs.

Rational design of high-yield and superior-quality rice

April 7th, 2017

Rice (Oryza sativa L.) is a staple food for more than half of the world’s population. To meet the ever-increasing demand for food, because of population growth and improved living standards, world rice production needs to double by 2030. The development of new elite rice varieties with high yield and superior quality is challenging for traditional breeding approaches, and new strategies need to be developed.

OsCUL3a Negatively Regulates Cell Death and Immunity by Degrading OsNPR1 in Rice

February 9th, 2017

The ubiquitin/proteasome pathway is a major system for the selective degradation of proteins in eukaryotes. Previous studies in plants have demonstrated that the ubiquitin/proteasome pathway is an essential component of the regulatory networks controlling many important cellular processes,

Regulatory Role of OsMADS34 in the Determination of Glumes Fate, Grain Yield, and Quality in Rice

January 23rd, 2017

In most flowering plants, flowers consist of sepals, petals, stamens, and pistils, which are arranged in concentric whorls. Genetic and morphological studies of floral homeotic mutants in eudicots have established the classical ABC model of floral development, and subsequently the ABCDE model, which can also partly explain floral development in grass species.

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Most Popular

· A simple and efficient method for CRISPR/Cas9-induced mutant screening

· Biomimetic sensors and biosensors for qualitative and quantitative analyses of five basic tastes

· Rational design of high-yield and superior-quality rice

· Regulatory Role of OsMADS34 in the Determination of Glumes Fate, Grain Yield, and Quality in Rice

· OsCUL3a Negatively Regulates Cell Death and Immunity by Degrading OsNPR1 in Rice

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