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A simple and efficient method for CRISPR/Cas9-induced mutant screening

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system provides a technological breakthrough in mutant generation. Several methods such as the polymerase chain reaction (PCR)/restriction enzyme (RE) assay, T7 endonuclease I (T7EI) assay, Surveyor nuclease assay, PAGE-based genotyping assay, and high-resolution melting (HRM) analysis-based assay have been developed for screening CRISPR/Cas9-induced mutants.

> Read the full story at Research Progress
Biomimetic sensors and biosensors for qualitative and quantitative analyses of five basic tastes

As is known to all, the basic taste includes sweetness, bitterness, sourness, saltiness and umami. The core of taste evaluation is the sense of taste. Sensory evaluation and chemical detection, as the methods of evaluating flavor quality, are commonly used in many products such as food stuffs, beverages and drugs.

> Read the full story at Research Progress
OsCUL3a Negatively Regulates Cell Death and Immunity by Degrading OsNPR1 in Rice

The ubiquitin/proteasome pathway is a major system for the selective degradation of proteins in eukaryotes. Previous studies in plants have demonstrated that the ubiquitin/proteasome pathway is an essential component of the regulatory networks controlling many important cellular processes,

> Read the full story at Research Progress
Regulatory Role of OsMADS34 in the Determination of Glumes Fate, Grain Yield, and Quality in Rice

In most flowering plants, flowers consist of sepals, petals, stamens, and pistils, which are arranged in concentric whorls. Genetic and morphological studies of floral homeotic mutants in eudicots have established the classical ABC model of floral development, and subsequently the ABCDE model, which can also partly explain floral development in grass species.

> Read the full story at Research Progress
Genome Editing in Rice Using CRISPR-Cpf1 System

Cpf1 is a class 2/typeV CRISPR effector that has been recently harnessed for genome editing in animal and human cell. Compared with CRIPSR-Cas9 system, Cpf1 recognizes thymidine-rich sequence as the protospacer-adjacent motif (PAM) at the 5’ end of target sequences (generally, 5’-TTTN).

> Read the full story at Research Progress
Fine mapping and candidate gene analysis of qHD5, a novel major QTL with pleiotropism for yield-related traits in rice (Oryza sativa L.)

Heading date (or flowering time, HD) represents one of the fundamental developmental events in the life cycle of the rice plant. It is one of the most important traits that enable rice to adapt to seasonal differences and specific growth conditions in diverse growing regions.

> Read the full story at Research Progress

· 1^st International Forum on Rice (Sanya, China)(1^st Announcement)

September 20^th, 2016

· Scientist Recruitment

April 29^th, 2016

· Enrolled in CNRRI’s Master/Ph.D. Program

November 12^th, 2014

· Work in CNRRI through Talented Young Scientist Program

October 28^th, 2014

· Rice Science Instruction for Contributors

October 28^th, 2014

· Talents Recruitment - Work in CNRRI

September 15^th, 2014

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